› 2e46

X-ray diffraction
2.3Å resolution

Crystal Structure Analysis of the clock protein EA4

Released:
DOI: 10.2210/pdb2e46/pdb
PDB entry 2e46 coloured by chain, front view.
PDB entry 2e46 coloured by chain, side view.
PDB entry 2e46 coloured by chain, top view.
Source organism: Bombyx mori
Entry authors: Park S-Y, Hiraki T

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170734 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Cu-Zn] Chain: A
Molecule details ›
Chain: A
Length: 157 amino acids
Theoretical weight: 16.77 KDa
Source organism: Bombyx mori
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q08J22 (Residues: 17-172; Coverage: 100%)
Gene names: 692772, ea4, time
Sequence domains: Copper/zinc superoxide dismutase (SODC)
Structure domains: Superoxide dismutase, copper/zinc binding domain

Ligands and Environments

2 bound ligands:
Ligand CU 2 x CU
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P41212
Unit cell:
a: 59.5Å b: 59.5Å c: 112.072Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.208 0.285
Expression system: Escherichia coli BL21(DE3)

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