› 2e48

X-ray diffraction
2.9Å resolution

Crystal Structure of Human D-Amino Acid Oxidase: Substrate-Free Holoenzyme

Released:
DOI: 10.2210/pdb2e48/pdb
PDB entry 2e48 coloured by chain, front view.
PDB entry 2e48 coloured by chain, side view.
PDB entry 2e48 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of D-DOPA oxidation by D-amino acid oxidase: alternative pathway for dopamine biosynthesis.
Kawazoe T, Tsuge H, Imagawa T, Aki K, Kuramitsu S, Fukui K
Biochem Biophys Res Commun 355 385-91 (2007)
PMID: 17303072

Function and Biology Details

Reaction catalysed: 
A D-amino acid + H(2)O + O(2) = a 2-oxo carboxylate + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-147153 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-amino-acid oxidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 347 amino acids
Theoretical weight: 39.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14920 (Residues: 1-347; Coverage: 100%)
Gene names: DAMOX, DAO
Sequence domains: FAD dependent oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21212
Unit cell:
a: 149.652Å b: 181.727Å c: 50.815Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.21 0.259
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

The neurobiology of D-amino acid oxidase and its involvement in schizophrenia.
Verrall et al. (2010)
8 more

8 mentions without citation

Human D-Amino Acid Oxidase: Structure, Function, and Regulation.
Pollegioni et al. (2018)
7 more