› 2ebn

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF ENDO-BETA-N-ACETYLGLUCOSAMINIDASE F1, AN ALPHA(SLASH)BETA-BARREL ENZYME ADAPTED FOR A COMPLEX SUBSTRATE

Released:
DOI: 10.2210/pdb2ebn/pdb
PDB entry 2ebn coloured by chain, front view.
PDB entry 2ebn coloured by chain, side view.
PDB entry 2ebn coloured by chain, top view.
Source organism: Elizabethkingia meningoseptica
Primary publication:
Crystal structure of endo-beta-N-acetylglucosaminidase F1, an alpha/beta-barrel enzyme adapted for a complex substrate.
Van Roey P, Rao V, Plummer TH, Tarentino AL
Biochemistry 33 13989-96 (1994)
PMID: 7947807

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)(2))Asn- structure. One N-acetyl-D-glucosamine residue remains attached to the protein, the rest of the oligosaccharide is released intact.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153256 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-beta-N-acetylglucosaminidase F1 Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 31.72 KDa
Source organism: Elizabethkingia meningoseptica
Expression system: Not provided
UniProt:
  • Canonical: P36911 (Residues: 51-339; Coverage: 100%)
Gene name: endOF1
Sequence domains: Glycosyl hydrolases family 18
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P61
Unit cell:
a: 70.64Å b: 70.64Å c: 103.31Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.158 0.158 not available
Expression system: Not provided

Quick links

Citations

6 review citations

Hyaluronidases--a group of neglected enzymes.
Kreil G. (1995)
5 more

11 mentions without citation

Sculpting therapeutic monoclonal antibody N-glycans using endoglycosidases.
Trastoy et al. (2022)
10 more