› 2ekc

X-ray diffraction
2Å resolution

Structural study of Project ID aq_1548 from Aquifex aeolicus VF5

Released:
DOI: 10.2210/pdb2ekc/pdb
PDB entry 2ekc coloured by chain, front view.
PDB entry 2ekc coloured by chain, side view.
PDB entry 2ekc coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Entry authors: Asada Y, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
(1a) 1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130501 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan synthase alpha chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 29.55 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O67502 (Residues: 1-262; Coverage: 100%)
Gene names: aq_1548, trpA
Sequence domains: Tryptophan synthase alpha chain
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 57.854Å b: 72.985Å c: 64.942Å
α: 90° β: 90.697° γ: 90°
R-values:
R R work R free
0.186 0.184 0.22
Expression system: Escherichia coli

Quick links