› 2emn

X-ray diffraction
2.3Å resolution

GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA, MUTANT

Released:
DOI: 10.2210/pdb2emn/pdb
PDB entry 2emn coloured by chain, front view.
PDB entry 2emn coloured by chain, side view.
PDB entry 2emn coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
The structural basis for spectral variations in green fluorescent protein.
Palm GJ, Zdanov A, Gaitanaris GA, Stauber R, Pavlakis GN, Wlodawer A
Nat Struct Biol 4 361-5 (1997)
PMID: 9145105

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154559 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.95 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CSH 1 x CSH

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P6122
Unit cell:
a: 89.2Å b: 89.2Å c: 130.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.198 0.299
Expression system: Escherichia coli

Quick links

Citations

18 review citations

The green fluorescent protein.
Tsien RY. (1998)
17 more