› 2eu0

Solution NMR

The NMR ensemble structure of the Itk SH2 domain bound to a phosphopeptide

Released:
DOI: 10.2210/pdb2eu0/pdb
PDB entry 2eu0 coloured by chain, ensemble of 20 models, front view.
PDB entry 2eu0 coloured by chain, ensemble of 20 models, side view.
PDB entry 2eu0 coloured by chain, ensemble of 20 models, top view.
Source organism: Mus musculus
Primary publication:
Molecular details of Itk activation by prolyl isomerization and phospholigand binding: the NMR structure of the Itk SH2 domain bound to a phosphopeptide.
Pletneva EV, Sundd M, Fulton DB, Andreotti AH
J Mol Biol 357 550-61 (2006)
PMID: 16436281

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169913 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase ITK/TSK Chain: A
Molecule details ›
Chain: A
Length: 109 amino acids
Theoretical weight: 12.56 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03526 (Residues: 238-344; Coverage: 17%)
Gene names: Emt, Itk, Tlk, Tsk
Sequence domains: SH2 domain
Structure domains: SH2 domain
Lymphocyte cytosolic protein 2 Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 795 Da
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q60787 (Residues: 143-148; Coverage: 1%)
Gene name: Lcp2

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
Refinement method: Distance geometry simulated annealing was used for refinement
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

8 review citations

T cell activation.
Smith-Garvin et al. (2009)
7 more