› 2ewk

X-ray diffraction
1Å resolution

The T24V mutant of tetraheme cytochrome c3 from Desulfovibrio Vulgaris Miyazaki F

Released:
DOI: 10.2210/pdb2ewk/pdb
PDB entry 2ewk coloured by chain, front view.
PDB entry 2ewk coloured by chain, side view.
PDB entry 2ewk coloured by chain, top view.
Source organism: Desulfovibrio vulgaris str. 'Miyazaki F'
Primary publication:
Strategic roles of axial histidines in structure formation and redox regulation of tetraheme cytochrome c3.
Takayama Y, Werbeck ND, Komori H, Morita K, Ozawa K, Higuchi Y, Akutsu H
Biochemistry 47 9405-15 (2008)
PMID: 18702516

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132281 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c3 Chain: A
Molecule details ›
Chain: A
Length: 107 amino acids
Theoretical weight: 11.56 KDa
Source organism: Desulfovibrio vulgaris str. 'Miyazaki F'
Expression system: Shewanella oneidensis
UniProt:
  • Canonical: P00132 (Residues: 24-130; Coverage: 100%)
Gene name: DvMF_2499
Sequence domains: Class III cytochrome C family
Structure domains: Cytochrome C3

Ligands and Environments

1 bound ligand:
Ligand HEM 4 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 52.425Å b: 67.537Å c: 34.412Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.112 0.11 0.14
Expression system: Shewanella oneidensis

Quick links

Citations

4 citation in other articles

Thermodynamic characterization of the redox centres in a representative domain of a novel c-type multihaem cytochrome.
Morgado et al. (2009)
3 more

2 mentions without citation

Structural Determination of a Filamentous Chaperone to Fabricate Electronically Conductive Metalloprotein Nanowires.
Chen et al. (2020)
1 more