› 2eyt

X-ray diffraction
2.6Å resolution

A structural basis for selection and cross-species reactivity of the semi-invariant NKT cell receptor in CD1d/glycolipid recognition

Released:
DOI: 10.2210/pdb2eyt/pdb
PDB entry 2eyt coloured by chain, front view.
PDB entry 2eyt coloured by chain, side view.
PDB entry 2eyt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A structural basis for selection and cross-species reactivity of the semi-invariant NKT cell receptor in CD1d/glycolipid recognition.
Kjer-Nielsen L, Borg NA, Pellicci DG, Beddoe T, Kostenko L, Clements CS, Williamson NA, Smyth MJ, Besra GS, Reid HH, Bharadwaj M, Godfrey DI, Rossjohn J, McCluskey J
J Exp Med 203 661-73 (2006)
PMID: 16505140

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-105335 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
T cell receptor alpha chain constant Chains: A, C
Molecule details ›
Chains: A, C
Length: 210 amino acids
Theoretical weight: 23.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01848 (Residues: 1-93; Coverage: 66%)
Gene names: TCRA, TRAC
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins
T cell receptor beta constant 2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 243 amino acids
Theoretical weight: 27.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A5B9 (Residues: 1-129; Coverage: 73%)
Gene names: TCRBC2, TRBC2
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P32
Unit cell:
a: 66.672Å b: 66.672Å c: 182.408Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.207 0.256
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

34 review citations

The biology of NKT cells.
Bendelac et al. (2007)
33 more

1 mention without citation

A structural basis for selection and cross-species reactivity of the semi-invariant NKT cell receptor in CD1d/glycolipid recognition.
Kjer-Nielsen et al. (2006)