› 2f2v

X-ray diffraction
1.85Å resolution

alpha-spectrin SH3 domain A56G mutant

Released:
DOI: 10.2210/pdb2f2v/pdb
PDB entry 2f2v coloured by chain, front view.
PDB entry 2f2v coloured by chain, side view.
PDB entry 2f2v coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Cooperative propagation of local stability changes from low-stability and high-stability regions in a SH3 domain.
Casares S, López-Mayorga O, Vega MC, Cámara-Artigas A, Conejero-Lara F
Proteins 67 531-47 (2007)
PMID: 17330285

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139741 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spectrin alpha chain, non-erythrocytic 1 Chain: A
Molecule details ›
Chain: A
Length: 62 amino acids
Theoretical weight: 7.22 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07751 (Residues: 965-1025; Coverage: 3%)
Gene names: SPTA2, SPTAN1
Sequence domains: SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

1 bound ligand:
Ligand FMT 2 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 23.932Å b: 41.617Å c: 45.981Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.223
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Interfacial water molecules in SH3 interactions: Getting the full picture on polyproline recognition by protein-protein interaction domains.
Zafra-Ruano et al. (2012)
1 more

2 mentions without citation

Conformational and functional analysis of molecular dynamics trajectories by self-organising maps.
Fraccalvieri et al. (2011)
1 more