› 2f8p

X-ray diffraction
1.93Å resolution

Crystal structure of obelin following Ca2+ triggered bioluminescence suggests neutral coelenteramide as the primary excited state

Released:
DOI: 10.2210/pdb2f8p/pdb
PDB entry 2f8p coloured by chain, front view.
PDB entry 2f8p coloured by chain, side view.
PDB entry 2f8p coloured by chain, top view.
Source organism: Obelia longissima
Primary publication:
Crystal structure of obelin after Ca2+-triggered bioluminescence suggests neutral coelenteramide as the primary excited state.
Liu ZJ, Stepanyuk GA, Vysotski ES, Lee J, Markova SV, Malikova NP, Wang BC
Proc Natl Acad Sci U S A 103 2570-5 (2006)
PMID: 16467137

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Obelin Chain: A
Molecule details ›
Chain: A
Length: 195 amino acids
Theoretical weight: 22.24 KDa
Source organism: Obelia longissima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27709 (Residues: 1-195; Coverage: 100%)
Sequence domains:
Structure domains: EF-hand

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand CEI 1 x CEI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43
Unit cell:
a: 64.26Å b: 64.26Å c: 63.049Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.23
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Coelenterazine-dependent luciferases.
Markova et al. (2015)
3 more

3 mentions without citation

Crystal structure of obelin after Ca2+-triggered bioluminescence suggests neutral coelenteramide as the primary excited state.
Liu et al. (2006)
2 more