2fgj

X-ray diffraction
2.6Å resolution

Crystal structure of the ABC-cassette H662A mutant of HlyB with bound ATP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-140359 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-hemolysin translocation ATP-binding protein HlyB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 247 amino acids
Theoretical weight: 27.69 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P08716 (Residues: 467-707; Coverage: 34%)
Gene name: hlyB
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P21
Unit cell:
a: 46.558Å b: 195.166Å c: 63.288Å
α: 90° β: 110.85° γ: 90°
R-values:
R R work R free
0.211 0.208 0.279
Expression system: Escherichia coli