› 2fhi

X-ray diffraction
2.6Å resolution

SUBSTRATE ANALOG (IB2) COMPLEX WITH THE HIS 96 ASN SUBSTITUTION OF THE FRAGILE HISTIDINE TRIAD PROTEIN, FHIT

Released:
DOI: 10.2210/pdb2fhi/pdb
PDB entry 2fhi coloured by chain, front view.
PDB entry 2fhi coloured by chain, side view.
PDB entry 2fhi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Genetic, biochemical, and crystallographic characterization of Fhit-substrate complexes as the active signaling form of Fhit.
Pace HC, Garrison PN, Robinson AK, Barnes LD, Draganescu A, Rösler A, Blackburn GM, Siprashvili Z, Croce CM, Huebner K, Brenner C
Proc Natl Acad Sci U S A 95 5484-9 (1998)
PMID: 9576908

Function and Biology Details

Reactions catalysed: 
Adenylyl sulfate + NH(3) = adenosine 5'-phosphoramidate + sulfate
P(1)-P(3)-bis(5'-adenosyl) triphosphate + H(2)O = ADP + AMP
Adenylyl sulfate + H(2)O = AMP + sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156029 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bis(5'-adenosyl)-triphosphatase Chain: A
Molecule details ›
Chain: A
Length: 147 amino acids
Theoretical weight: 16.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49789 (Residues: 1-147; Coverage: 100%)
Gene name: FHIT
Sequence domains: HIT domain
Structure domains: HIT-like

Ligands and Environments

1 bound ligand:
Ligand IB2 1 x IB2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P6122
Unit cell:
a: 50.46Å b: 50.46Å c: 268.95Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.241 0.241 0.278
Expression system: Escherichia coli

Quick links

Citations

17 review citations

FRA3B and other common fragile sites: the weakest links.
Huebner et al. (2001)
16 more

2 mentions without citation

Genetic, biochemical, and crystallographic characterization of Fhit-substrate complexes as the active signaling form of Fhit.
Pace et al. (1998)
1 more