› 2fka

X-ray diffraction
2Å resolution

Crystal structure of Mg(2+) and BeF(3)(-)-bound CheY in complex with CheZ(200-214) solved from a F432 crystal grown in CAPS (pH 10.5)

Released:
DOI: 10.2210/pdb2fka/pdb
PDB entry 2fka coloured by chain, front view.
PDB entry 2fka coloured by chain, side view.
PDB entry 2fka coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Crystal structures of beryllium fluoride-free and beryllium fluoride-bound CheY in complex with the conserved C-terminal peptide of CheZ reveal dual binding modes specific to CheY conformation.
Guhaniyogi J, Robinson VL, Stock AM
J Mol Biol 359 624-45 (2006)
PMID: 16674976

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
homo dodecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-139761 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chemotaxis protein CheY Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.14 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A2D5 (Residues: 1-129; Coverage: 100%)
Gene names: STM1916, cheY
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold
Protein phosphatase CheZ Chain: B
Molecule details ›
Chain: B
Length: 15 amino acids
Theoretical weight: 1.62 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Not provided
UniProt:
  • Canonical: P07800 (Residues: 200-214; Coverage: 7%)
Gene names: STM1915, cheZ

Ligands and Environments

4 bound ligands:
Ligand MG 1 x MG
Ligand SO4 1 x SO4
Ligand CXS 2 x CXS
Ligand BEF 1 x BEF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: F432
Unit cell:
a: 198.73Å b: 198.73Å c: 198.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.224
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Bacterial response regulators: versatile regulatory strategies from common domains.
Gao et al. (2007)
1 more

6 mentions without citation

Benchmarking of different molecular docking methods for protein-peptide docking.
Agrawal et al. (2019)
5 more