› 2fl0

X-ray diffraction
2.7Å resolution

Oxidized (All ferric) form of the Azotobacter vinelandii bacterioferritin

Released:
DOI: 10.2210/pdb2fl0/pdb
PDB entry 2fl0 coloured by chain, front view.
PDB entry 2fl0 coloured by chain, side view.
PDB entry 2fl0 coloured by chain, top view.
Source organism: Azotobacter vinelandii
Primary publication:
Redox-dependent structural changes in the Azotobacter vinelandii bacterioferritin: new insights into the ferroxidase and iron transport mechanism.
Swartz L, Kuchinskas M, Li H, Poulos TL, Lanzilotta WN
Biochemistry 45 4421-8 (2006)
PMID: 16584178

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149796 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterioferritin Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 155 amino acids
Theoretical weight: 18 KDa
Source organism: Azotobacter vinelandii
UniProt:
  • Canonical: P22759 (Residues: 1-155; Coverage: 99%)
Gene name: bfr
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

3 bound ligands:
Ligand FE2 18 x FE2
Ligand MG 13 x MG
Ligand HEM 4 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: R3
Unit cell:
a: 124.48Å b: 124.48Å c: 285.112Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.203 0.256

Quick links

Citations

4 review citations

Ferritins: furnishing proteins with iron.
Bradley et al. (2016)
3 more

3 mentions without citation

Crystal structure of Bfr A from Mycobacterium tuberculosis: incorporation of selenomethionine results in cleavage and demetallation of haem.
Gupta et al. (2009)
2 more