› 2fmy

X-ray diffraction
2.2Å resolution

CO-dependent transcription factor CooA from Carboxydothermus hydrogenoformans (Imidazole-bound form)

Released:
DOI: 10.2210/pdb2fmy/pdb
PDB entry 2fmy coloured by chain, front view.
PDB entry 2fmy coloured by chain, side view.
PDB entry 2fmy coloured by chain, top view.
Source organism: Carboxydothermus hydrogenoformans
Primary publication:
Crystal structure of CO-sensing transcription activator CooA bound to exogenous ligand imidazole.
Komori H, Inagaki S, Yoshioka S, Aono S, Higuchi Y
J Mol Biol 367 864-71 (2007)
PMID: 17292914

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174495 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbon monoxide oxidation system transcription regulator CooA-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 220 amino acids
Theoretical weight: 25.09 KDa
Source organism: Carboxydothermus hydrogenoformans
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q3AB29 (Residues: 1-217; Coverage: 100%)
Gene names: CHY_1835, cooA-1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand HEM 4 x HEM
Ligand IMD 4 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P21
Unit cell:
a: 61.654Å b: 93.93Å c: 91.901Å
α: 90° β: 104.73° γ: 90°
R-values:
R R work R free
0.209 0.207 0.247
Expression system: Escherichia coli BL21

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Citations

8 review citations

'That which does not kill us only makes us stronger': the role of carbon monoxide in thermophilic microbial consortia.
Techtmann et al. (2009)
7 more

1 mention without citation

Vibrational coherence spectroscopy of the heme domain in the CO-sensing transcriptional activator CooA.
Karunakaran et al. (2011)