› 2fo0

X-ray diffraction
2.27Å resolution

Organization of the SH3-SH2 Unit in Active and Inactive Forms of the c-Abl Tyrosine Kinase

Released:
DOI: 10.2210/pdb2fo0/pdb
PDB entry 2fo0 coloured by chain, front view.
PDB entry 2fo0 coloured by chain, side view.
PDB entry 2fo0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Organization of the SH3-SH2 unit in active and inactive forms of the c-Abl tyrosine kinase.
Nagar B, Hantschel O, Seeliger M, Davies JM, Weis WI, Superti-Furga G, Kuriyan J
Mol Cell 21 787-98 (2006)
PMID: 16543148

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132602 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase ABL1 Chain: A
Molecule details ›
Chain: A
Length: 495 amino acids
Theoretical weight: 56.45 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00519 (Residues: 38-512; Coverage: 42%)
Gene names: ABL, ABL1, JTK7
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MYR 1 x MYR
Ligand P16 1 x P16
Ligand GOL 2 x GOL
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21212
Unit cell:
a: 79.688Å b: 117.266Å c: 60.416Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.246
Expression system: Spodoptera frugiperda

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Citations

25 review citations

X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution.
Putnam et al. (2007)
24 more

44 mentions without citation

Mechanisms of drug resistance in kinases.
Barouch-Bentov et al. (2011)
43 more