› 2fo4

X-ray diffraction
2.7Å resolution

Enhanced MHC class I binding and immune responses through anchor modification of the non-canonical tumor associated MUC1-8 peptide

Released:
DOI: 10.2210/pdb2fo4/pdb
PDB entry 2fo4 coloured by chain, front view.
PDB entry 2fo4 coloured by chain, side view.
PDB entry 2fo4 coloured by chain, top view.
Source organisms:
Primary publication:
Enhanced major histocompatibility complex class I binding and immune responses through anchor modification of the non-canonical tumour-associated mucin 1-8 peptide.
Lazoura E, Lodding J, Farrugia W, Ramsland PA, Stevens J, Wilson IA, Pietersz GA, Apostolopoulos V
Immunology 119 306-16 (2006)
PMID: 17067310

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135107 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.65 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q3UBW0 (Residues: 22-295; Coverage: 84%)
Gene name: H2-K1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Mucin-1 subunit alpha Chain: P
Molecule details ›
Chain: P
Length: 8 amino acids
Theoretical weight: 903 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P15941 (Residues: 920-927; Coverage: 1%)
Gene names: MUC1, PUM

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
3 bound ligands:
Ligand NAG 1 x NAG
Ligand PO4 2 x PO4
Ligand MPD 4 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21212
Unit cell:
a: 135.26Å b: 87.89Å c: 45.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.248
Expression systems:
  • Drosophila melanogaster
  • Not provided

Quick links

Citations

6 review citations

Computer-aided biotechnology: from immuno-informatics to reverse vaccinology.
Vivona et al. (2008)
5 more