› 2fok

X-ray diffraction
2.3Å resolution

STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI

Released:
DOI: 10.2210/pdb2fok/pdb
PDB entry 2fok coloured by chain, front view.
PDB entry 2fok coloured by chain, side view.
PDB entry 2fok coloured by chain, top view.
Source organism: Planomicrobium okeanokoites
Primary publication:
Structure of FokI has implications for DNA cleavage.
Wah DA, Bitinaite J, Schildkraut I, Aggarwal AK
Proc Natl Acad Sci U S A 95 10564-9 (1998)
PMID: 9724743

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II restriction enzyme FokI Chains: A, B
Molecule details ›
Chains: A, B
Length: 579 amino acids
Theoretical weight: 65.83 KDa
Source organism: Planomicrobium okeanokoites
Expression system: Escherichia coli
UniProt:
  • Canonical: P14870 (Residues: 1-579; Coverage: 100%)
Gene names: fokIR, rfoKI
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 54.01Å b: 137.17Å c: 188.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.211 0.306
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Structure and function of type II restriction endonucleases.
Pingoud et al. (2001)
24 more

19 mentions without citation

Genome engineering with zinc-finger nucleases.
Carroll D. (2011)
18 more