2fqz

X-ray diffraction
2Å resolution

Metal-depleted Ecl18kI in complex with uncleaved DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-115274 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Restriction endonuclease type II EcoRII C-terminal domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 305 amino acids
Theoretical weight: 35.94 KDa
Source organism: Enterobacter cloacae
Expression system: Escherichia coli
UniProt:
  • Canonical: O87963 (Residues: 1-305; Coverage: 100%)
Gene name: ecl18kIR
Sequence domains: EcoRII C terminal
Structure domains: Restriction Endonuclease
DNA STRAND 1 Chains: E, G
Molecule details ›
Chains: E, G
Length: 9 nucleotides
Theoretical weight: 2.74 KDa
Source organism: Enterobacter cloacae
Expression system: Not provided
DNA STRAND 2 Chains: F, H
Molecule details ›
Chains: F, H
Length: 9 nucleotides
Theoretical weight: 2.73 KDa
Source organism: Enterobacter cloacae
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13, MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P212121
Unit cell:
a: 77.371Å b: 95.519Å c: 190.173Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.21 0.26
Expression systems:
  • Escherichia coli
  • Not provided