› 2fvy

X-ray diffraction
0.92Å resolution

High Resolution Glucose Bound Crystal Structure of GGBP

Released:
DOI: 10.2210/pdb2fvy/pdb
PDB entry 2fvy coloured by chain, front view.
PDB entry 2fvy coloured by chain, side view.
PDB entry 2fvy coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Conformational changes of glucose/galactose-binding protein illuminated by open, unliganded, and ultra-high-resolution ligand-bound structures.
Borrok MJ, Kiessling LL, Forest KT
Protein Sci 16 1032-41 (2007)
PMID: 17473016

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-galactose/methyl-galactoside binding periplasmic protein MglB Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 33.41 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEE5 (Residues: 24-332; Coverage: 100%)
Gene names: JW2137, b2150, mglB
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

5 bound ligands:
Ligand BGC 1 x BGC
Ligand CA 1 x CA
Ligand ACT 1 x ACT
Ligand CO2 1 x CO2
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: C2
Unit cell:
a: 119.989Å b: 36.238Å c: 80.101Å
α: 90° β: 124.5° γ: 90°
R-values:
R R work R free
0.11 0.11 0.13
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Overview of fluorescence glucose sensing: a technology with a bright future.
Klonoff DC. (2012)
5 more

21 mentions without citation

Macromolecular ab initio phasing enforcing secondary and tertiary structure.
Millán et al. (2015)
20 more