› 2fw0

X-ray diffraction
1.55Å resolution

Apo Open Form of Glucose/Galactose Binding Protein

Released:
DOI: 10.2210/pdb2fw0/pdb
PDB entry 2fw0 coloured by chain, front view.
PDB entry 2fw0 coloured by chain, side view.
PDB entry 2fw0 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Conformational changes of glucose/galactose-binding protein illuminated by open, unliganded, and ultra-high-resolution ligand-bound structures.
Borrok MJ, Kiessling LL, Forest KT
Protein Sci 16 1032-41 (2007)
PMID: 17473016

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-galactose/methyl-galactoside binding periplasmic protein MglB Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 33.41 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEE5 (Residues: 24-332; Coverage: 100%)
Gene names: JW2137, b2150, mglB
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

4 bound ligands:
Ligand CA 1 x CA
Ligand NA 3 x NA
Ligand CIT 1 x CIT
Ligand MLA 1 x MLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 56.8Å b: 70.3Å c: 112.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.165 0.178
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Overview of fluorescence glucose sensing: a technology with a bright future.
Klonoff DC. (2012)
5 more

17 mentions without citation

Engineering genetically encoded FRET sensors.
Lindenburg et al. (2014)
16 more