› 2g3b

X-ray diffraction
2Å resolution

Crystal structure of putative TetR-family transcriptional regulator from Rhodococcus sp.

Released:
DOI: 10.2210/pdb2g3b/pdb
PDB entry 2g3b coloured by chain, front view.
PDB entry 2g3b coloured by chain, side view.
PDB entry 2g3b coloured by chain, top view.
Source organism: Rhodococcus jostii RHA1
Entry authors: Chruszcz M, Evdokimova E, Cymborowski M, Kagan O, Wang S, Koclega KD, Savchenko A, Edwards A, Joachimiak A, Minor W, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171125 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 208 amino acids
Theoretical weight: 22.9 KDa
Source organism: Rhodococcus jostii RHA1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0S8G3 (Residues: 1-208; Coverage: 100%)
Gene name: RHA1_ro04387
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 53.89Å b: 75.32Å c: 100.248Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.24
Expression system: Escherichia coli

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