› 2g9h

X-ray diffraction
2Å resolution

Crystal Structure of Staphylococcal Enterotoxin I (SEI) in Complex with a Human MHC class II Molecule

Released:
DOI: 10.2210/pdb2g9h/pdb
PDB entry 2g9h coloured by chain, front view.
PDB entry 2g9h coloured by chain, side view.
PDB entry 2g9h coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of staphylococcal enterotoxin I (SEI) in complex with a human major histocompatibility complex class II molecule.
Fernández MM, Guan R, Swaminathan CP, Malchiodi EL, Mariuzza RA
J Biol Chem 281 25356-64 (2006)
PMID: 16829512

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-135321 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
HLA class II histocompatibility antigen, DR alpha chain Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 21.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01903 (Residues: 26-207; Coverage: 80%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DRB1 beta chain Chain: B
Molecule details ›
Chain: B
Length: 190 amino acids
Theoretical weight: 22.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01911 (Residues: 30-219; Coverage: 80%)
Gene name: HLA-DRB1
Sequence domains:
Structure domains:
Hemagglutinin HA1 chain Chain: C
Molecule details ›
Chain: C
Length: 13 amino acids
Theoretical weight: 1.51 KDa
Source organism: Influenza A virus (A/England/878/1969(H3N2))
Expression system: Not provided
UniProt:
  • Canonical: P04664 (Residues: 306-318; Coverage: 4%)
Gene name: HA
Enterotoxin type I Chain: D
Molecule details ›
Chain: D
Length: 218 amino acids
Theoretical weight: 24.96 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: Q52T95 (Residues: 1-218; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand SO4 4 x SO4
Ligand EPE 1 x EPE
Ligand DIO 2 x DIO
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 150.395Å b: 99.933Å c: 72.917Å
α: 90° β: 92° γ: 90°
R-values:
R R work R free
0.215 0.213 0.252
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

10 review citations

Staphylococcus aureus and its food poisoning toxins: characterization and outbreak investigation.
Hennekinne et al. (2012)
9 more

25 mentions without citation

Conformational variation in structures of classical and non-classical MHCII proteins and functional implications.
Painter et al. (2012)
24 more