› 2gb0

X-ray diffraction
1.85Å resolution

Monomeric sarcosine oxidase: structure of a covalently flavinylated amine oxidizing enzyme

Released:
DOI: 10.2210/pdb2gb0/pdb
PDB entry 2gb0 coloured by chain, front view.
PDB entry 2gb0 coloured by chain, side view.
PDB entry 2gb0 coloured by chain, top view.
Source organism: Bacillus sp. B-0618
Primary publication:
Monomeric sarcosine oxidase: structure of a covalently flavinylated amine oxidizing enzyme.
Trickey P, Wagner MA, Jorns MS, Mathews FS
Structure 7 331-45 (1999)
PMID: 10368302

Function and Biology Details

Reaction catalysed: 
Sarcosine + H(2)O + O(2) = glycine + formaldehyde + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monomeric sarcosine oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 389 amino acids
Theoretical weight: 43.1 KDa
Source organism: Bacillus sp. B-0618
Expression system: Escherichia coli
UniProt:
  • Canonical: P40859 (Residues: 2-390; Coverage: 100%)
Gene names: sox, soxA
Sequence domains: FAD dependent oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
2 bound ligands:
Ligand PO4 4 x PO4
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 72.562Å b: 69.555Å c: 74.117Å
α: 90° β: 94.26° γ: 90°
R-values:
R R work R free
0.165 0.165 0.198
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Sequence-structure analysis of FAD-containing proteins.
Dym et al. (2001)
10 more

8 mentions without citation

CASP9 assessment of free modeling target predictions.
Kinch et al. (2011)
7 more