› 2gdv

X-ray diffraction
2Å resolution

Sucrose phosphorylase from BIFIDOBACTERIUM ADOLESCENTIS reacted with sucrose

Released:
DOI: 10.2210/pdb2gdv/pdb
PDB entry 2gdv coloured by chain, front view.
PDB entry 2gdv coloured by chain, side view.
PDB entry 2gdv coloured by chain, top view.
Source organism: Bifidobacterium adolescentis
Entry authors: Skov LK, Mirza O, Gajhede M, Katsrup JS

Function and Biology Details

Reaction catalysed: 
Sucrose + phosphate = D-fructose + alpha-D-glucose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106526 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sucrose phosphorylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 504 amino acids
Theoretical weight: 56.25 KDa
Source organism: Bifidobacterium adolescentis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0ZZH6 (Residues: 1-504; Coverage: 100%)
Gene names: BAD_0078, spl, sucP
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BGC 2 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 75.74Å b: 103.07Å c: 150.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.237
Expression system: Escherichia coli

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Citations

6 review citations

Bifidobacterium carbohydrases-their role in breakdown and synthesis of (potential) prebiotics.
van den Broek et al. (2008)
5 more

5 mentions without citation

Crystal structure of a metal-dependent phosphoesterase (YP_910028.1) from Bifidobacterium adolescentis: Computational prediction and experimental validation of phosphoesterase activity.
Han et al. (2011)
4 more