› 2gew

X-ray diffraction
0.97Å resolution

Atomic resolution structure of cholesterol oxidase @ pH 9.0 (Streptomyces sp. SA-COO)

Released:
DOI: 10.2210/pdb2gew/pdb
PDB entry 2gew coloured by chain, front view.
PDB entry 2gew coloured by chain, side view.
PDB entry 2gew coloured by chain, top view.
Source organism: Streptomyces sp. SA-COO
Primary publication:
Atomic resolution crystallography reveals how changes in pH shape the protein microenvironment.
Lyubimov AY, Lario PI, Moustafa I, Vrielink A
Nat Chem Biol 2 259-64 (2006)
PMID: 16604066

Function and Biology Details

Reactions catalysed: 
Cholesterol + O(2) = cholest-5-en-3-one + H(2)O(2)
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146297 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholesterol oxidase Chain: A
Molecule details ›
Chain: A
Length: 504 amino acids
Theoretical weight: 54.97 KDa
Source organism: Streptomyces sp. SA-COO
Expression system: Escherichia coli
UniProt:
  • Canonical: P12676 (Residues: 43-546; Coverage: 100%)
Gene name: choA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
2 bound ligands:
Ligand SO4 1 x SO4
Ligand OXY 1 x OXY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 51.11Å b: 73.256Å c: 62.432Å
α: 90° β: 104.05° γ: 90°
R-values:
R R work R free
0.113 0.113 0.14
Expression system: Escherichia coli

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Citations

5 review citations

Fungal aryl-alcohol oxidase: a peroxide-producing flavoenzyme involved in lignin degradation.
Hernández-Ortega et al. (2012)
4 more

4 mentions without citation

Cloning, expression, and in silico structural modeling of cholesterol oxidase of Acinetobacter sp. strain RAMD in E. coli.
Mahmoud et al. (2021)
3 more