› 2gli

X-ray diffraction
2.6Å resolution

FIVE-FINGER GLI/DNA COMPLEX

Released:
DOI: 10.2210/pdb2gli/pdb
PDB entry 2gli coloured by chain, front view.
PDB entry 2gli coloured by chain, side view.
PDB entry 2gli coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a five-finger GLI-DNA complex: new perspectives on zinc fingers.
Pavletich NP, Pabo CO
Science 261 1701-7 (1993)
PMID: 8378770

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115304 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Zinc finger protein GLI1 Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 18.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08151 (Residues: 234-388; Coverage: 14%)
Gene names: GLI, GLI1
Sequence domains: Zinc finger, C2H2 type
Structure domains: Classic Zinc Finger
DNA (5'-D(*TP*TP*TP*CP*GP*TP*CP*TP*TP*GP*GP*GP*TP*GP*GP*TP*CP*CP*AP*CP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.45 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*AP*CP*GP*TP*GP*GP*AP*CP*CP*AP*CP*CP*CP*AP*AP*GP*AP*CP*GP*AP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.44 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CO 5 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 149Å b: 50.2Å c: 45.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 not available not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

62 review citations

Genome editing with engineered zinc finger nucleases.
Urnov et al. (2010)
61 more

36 mentions without citation

Context-dependent signal integration by the GLI code: the oncogenic load, pathways, modifiers and implications for cancer therapy.
Aberger et al. (2014)
35 more