› 2gpc

X-ray diffraction
1.9Å resolution

The crystal structure of the enzyme Fe-superoxide dismutase from Trypanosoma cruzi

Released:
DOI: 10.2210/pdb2gpc/pdb
PDB entry 2gpc coloured by chain, front view.
PDB entry 2gpc coloured by chain, side view.
PDB entry 2gpc coloured by chain, top view.
Source organism: Trypanosoma cruzi
Primary publication:
Systematic structural studies of iron superoxide dismutases from human parasites and a statistical coupling analysis of metal binding specificity.
Bachega JF, Navarro MV, Bleicher L, Bortoleto-Bugs RK, Dive D, Hoffmann P, Viscogliosi E, Garratt RC
Proteins 77 26-37 (2009)
PMID: 19384994

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175473 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase Chains: A, B
Molecule details ›
Chains: A, B
Length: 194 amino acids
Theoretical weight: 21.88 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4DI29 (Residues: 2-195; Coverage: 100%)
Gene name: Tc00.1047053511019.90
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand FE2 2 x FE2
Ligand MG 1 x MG
1 modified residue:
Ligand OCS 2 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 109.626Å b: 38.985Å c: 91.073Å
α: 90° β: 105.85° γ: 90°
R-values:
R R work R free
0.157 0.154 0.198
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Superoxide dismutases and superoxide reductases.
Sheng et al. (2014)
3 more

8 mentions without citation

Chemical Warfare at the Microorganismal Level: A Closer Look at the Superoxide Dismutase Enzymes of Pathogens.
Schatzman et al. (2018)
7 more