› 2gq3

X-ray diffraction
2.3Å resolution

mycobacterium tuberculosis malate synthase in complex with magnesium, malate, and coenzyme A

Released:
DOI: 10.2210/pdb2gq3/pdb
PDB entry 2gq3 coloured by chain, front view.
PDB entry 2gq3 coloured by chain, side view.
PDB entry 2gq3 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
The product complex of M. tuberculosis malate synthase revisited.
Anstrom DM, Remington SJ
Protein Sci 15 2002-7 (2006)
PMID: 16877713

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + H(2)O + glyoxylate = (S)-malate + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate synthase G Chains: A, B
Molecule details ›
Chains: A, B
Length: 729 amino acids
Theoretical weight: 79.22 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WK17 (Residues: 1-727; Coverage: 98%)
Gene names: MTCY1A11.06, Rv1837c, glcB
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand COA 1 x COA
3 bound ligands:
Ligand MG 4 x MG
Ligand MLT 2 x MLT
Ligand EPE 2 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P41212
Unit cell:
a: 120.38Å b: 120.38Å c: 238.889Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.178 0.244
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The TB Structural Genomics Consortium: a decade of progress.
Chim et al. (2011)
1 more

9 mentions without citation

Computational systems chemical biology.
Oprea et al. (2011)
8 more