› 2gqw

X-ray diffraction
1.4Å resolution

Crystal structure of Ferredoxin reductase, BphA4 (oxidized form)

Released:
DOI: 10.2210/pdb2gqw/pdb
PDB entry 2gqw coloured by chain, front view.
PDB entry 2gqw coloured by chain, side view.
PDB entry 2gqw coloured by chain, top view.
Source organism: Pseudomonas sp. KKS102
Primary publication:
Molecular mechanism of the redox-dependent interaction between NADH-dependent ferredoxin reductase and Rieske-type [2Fe-2S] ferredoxin.
Senda M, Kishigami S, Kimura S, Fukuda M, Ishida T, Senda T
J Mol Biol 373 382-400 (2007)
PMID: 17850818

Function and Biology Details

Reaction catalysed: 
2 reduced ferredoxin + NADP(+) + H(+) = 2 oxidized ferredoxin + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176010 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferredoxin reductase Chain: A
Molecule details ›
Chain: A
Length: 408 amino acids
Theoretical weight: 43.22 KDa
Source organism: Pseudomonas sp. KKS102
Expression system: Escherichia coli
UniProt:
  • Canonical: Q52437 (Residues: 1-408; Coverage: 100%)
Gene name: bphA4
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
2 bound ligands:
Ligand GOL 1 x GOL
Ligand FMT 5 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P6122
Unit cell:
a: 98.409Å b: 98.409Å c: 170.582Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.178 0.196
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Non-heme iron-dependent dioxygenases: unravelling catalytic mechanisms for complex enzymatic oxidations.
Bugg et al. (2008)
8 more

3 mentions without citation

Variants in the Oxidoreductase PYROXD1 Cause Early-Onset Myopathy with Internalized Nuclei and Myofibrillar Disorganization.
O'Grady et al. (2016)
2 more