› 2gs7

X-ray diffraction
2.6Å resolution

Crystal Structure of the inactive EGFR kinase domain in complex with AMP-PNP

Released:
DOI: 10.2210/pdb2gs7/pdb
PDB entry 2gs7 coloured by chain, front view.
PDB entry 2gs7 coloured by chain, side view.
PDB entry 2gs7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An allosteric mechanism for activation of the kinase domain of epidermal growth factor receptor.
Zhang X, Gureasko J, Shen K, Cole PA, Kuriyan J
Cell 125 1137-49 (2006)
PMID: 16777603

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Epidermal growth factor receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 37.62 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00533 (Residues: 696-1022; Coverage: 28%)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand IOD 11 x IOD
Ligand ANP 2 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 64.601Å b: 85.253Å c: 78.327Å
α: 90° β: 96.36° γ: 90°
R-values:
R R work R free
0.258 0.233 0.298
Expression system: Spodoptera frugiperda

Quick links

Citations

265 review citations

Cell signaling by receptor tyrosine kinases.
Lemmon et al. (2010)
264 more

64 mentions without citation

The EGFR family: not so prototypical receptor tyrosine kinases.
Lemmon et al. (2014)
63 more