› 2gsq

X-ray diffraction
2.2Å resolution

GLUTATHIONE S-TRANSFERASE FROM SQUID DIGESTIVE GLAND COMPLEXED WITH S-(3-IODOBENZYL)GLUTATHIONE

Released:
DOI: 10.2210/pdb2gsq/pdb
PDB entry 2gsq coloured by chain, front view.
PDB entry 2gsq coloured by chain, side view.
PDB entry 2gsq coloured by chain, top view.
Source organism: Nototodarus sloanii
Primary publication:
Location of a potential transport binding site in a sigma class glutathione transferase by x-ray crystallography.
Ji X, von Rosenvinge EC, Johnson WW, Armstrong RN, Gilliland GL
Proc Natl Acad Sci U S A 93 8208-13 (1996)
PMID: 8710848

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155424 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase Chain: A
Molecule details ›
Chain: A
Length: 202 amino acids
Theoretical weight: 22.82 KDa
Source organism: Nototodarus sloanii
Expression system: Escherichia coli
UniProt:
  • Canonical: P46088 (Residues: 2-203; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GBI 2 x GBI
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3121
Unit cell:
a: 73.15Å b: 73.15Å c: 94.88Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 not available not available
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamily.
Sheehan et al. (2001)
3 more

5 mentions without citation

Glutathione transferases are structural and functional outliers in the thioredoxin fold.
Atkinson et al. (2009)
4 more