› 2gtm

X-ray diffraction
1.9Å resolution

Mutated Mouse P38 MAP Kinase Domain in complex with Inhibitor PG-892579

Released:
DOI: 10.2210/pdb2gtm/pdb
PDB entry 2gtm coloured by chain, front view.
PDB entry 2gtm coloured by chain, side view.
PDB entry 2gtm coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The development of novel C-2, C-8, and N-9 trisubstituted purines as inhibitors of TNF-alpha production.
Sabat M, Vanrens JC, Clark MP, Brugel TA, Maier J, Bookland RG, Laufersweiler MJ, Laughlin SK, Golebiowski A, De B, Hsieh LC, Walter RL, Mekel MJ, Janusz MJ
Bioorg Med Chem Lett 16 4360-5 (2006)
PMID: 16750367

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155650 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase 14 Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 39.9 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P47811 (Residues: 5-352; Coverage: 97%)
Gene names: Crk1, Csbp1, Csbp2, Mapk14
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand LID 1 x LID
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 63.876Å b: 73.627Å c: 76.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.212 0.261
Expression system: Escherichia coli BL21(DE3)

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Citations

5 citation in other articles

Development of Novel In Vivo Chemical Probes to Address CNS Protein Kinase Involvement in Synaptic Dysfunction.
Watterson et al. (2013)
4 more

2 mentions without citation

p38α Mitogen-Activated Protein Kinase Is a Druggable Target in Pancreatic Adenocarcinoma.
Yang et al. (2019)
1 more