› 2h08

X-ray diffraction
2.5Å resolution

crystal structure of human phosphoribosyl pyrophosphate synthetase 1 mutant Y146M

Released:
DOI: 10.2210/pdb2h08/pdb
PDB entry 2h08 coloured by chain, front view.
PDB entry 2h08 coloured by chain, side view.
PDB entry 2h08 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human phosphoribosylpyrophosphate synthetase 1 reveals a novel allosteric site.
Li S, Lu Y, Peng B, Ding J
Biochem J 401 39-47 (2007)
PMID: 16939420

Function and Biology Details

Reaction catalysed: 
ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158121 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribose-phosphate pyrophosphokinase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 326 amino acids
Theoretical weight: 35.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60891 (Residues: 1-318; Coverage: 100%)
Gene name: PRPS1
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: R3
Unit cell:
a: 169.914Å b: 169.914Å c: 61.706Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.207 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Oxyl radicals, redox-sensitive signalling cascades and antioxidants.
Genestra M. (2007)
6 more

2 mentions without citation

Phosphoribosyl Diphosphate (PRPP): Biosynthesis, Enzymology, Utilization, and Metabolic Significance.
Hove-Jensen et al. (2017)
1 more