2h1k

X-ray diffraction
2.42Å resolution

Crystal structure of the Pdx1 homeodomain in complex with DNA

Released:
Source organism: Mesocricetus auratus

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-115321 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Pancreas/duodenum homeobox protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 63 amino acids
Theoretical weight: 7.89 KDa
Source organism: Mesocricetus auratus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P70118 (Residues: 146-206; Coverage: 22%)
Gene names: IPF1, PDX1
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
15-mer DNA Chains: C, E
Molecule details ›
Chains: C, E
Length: 15 nucleotides
Theoretical weight: 4.63 KDa
Source organism: Mesocricetus auratus
Expression system: Not provided
15-mer DNA Chains: D, F
Molecule details ›
Chains: D, F
Length: 15 nucleotides
Theoretical weight: 4.55 KDa
Source organism: Mesocricetus auratus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 58.282Å b: 61.928Å c: 96.445Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.277
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided