› 2h32

X-ray diffraction
2.7Å resolution

Crystal structure of the pre-B cell receptor

Released:
DOI: 10.2210/pdb2h32/pdb
PDB entry 2h32 coloured by chain, front view.
PDB entry 2h32 coloured by chain, side view.
PDB entry 2h32 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insight into pre-B cell receptor function.
Bankovich AJ, Raunser S, Juo ZS, Walz T, Davis MM, Garcia KC
Science 316 291-4 (2007)
PMID: 17431183

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
PDBe Complex ID:
PDB-CPX-210517 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin iota chain Chain: A
Molecule details ›
Chain: A
Length: 126 amino acids
Theoretical weight: 14.55 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P12018 (Residues: 20-145; Coverage: 100%)
Gene names: VPREB, VPREB1
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Immunoglobulin lambda-like polypeptide 1 Chain: B
Molecule details ›
Chain: B
Length: 121 amino acids
Theoretical weight: 13.13 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P15814 (Residues: 93-213; Coverage: 69%)
Gene names: IGL1, IGLL1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Immunoglobulin heavy chain Chain: H
Molecule details ›
Chain: H
Length: 223 amino acids
Theoretical weight: 24.5 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P43212
Unit cell:
a: 71.502Å b: 71.502Å c: 217.932Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.269 0.267 0.295
Expression system: Trichoplusia ni

Quick links

Citations

24 review citations

Nanobodies: natural single-domain antibodies.
Muyldermans S. (2013)
23 more

12 mentions without citation

Blind prediction performance of RosettaAntibody 3.0: grafting, relaxation, kinematic loop modeling, and full CDR optimization.
Weitzner et al. (2014)
11 more