› 2h3o

Solid-state NMR

Structure of MERFT, a membrane protein with two trans-membrane helices

Released:
DOI: 10.2210/pdb2h3o/pdb
PDB entry 2h3o coloured by chain, front view.
PDB entry 2h3o coloured by chain, side view.
PDB entry 2h3o coloured by chain, top view.
Source organism: Morganella morganii
Primary publication:
Structure determination of a membrane protein with two trans-membrane helices in aligned phospholipid bicelles by solid-state NMR spectroscopy.
De Angelis AA, Howell SC, Nevzorov AA, Opella SJ
J Am Chem Soc 128 12256-67 (2006)
PMID: 16967977

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176373 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mercury resistance system transport protein MerF Chain: A
Molecule details ›
Chain: A
Length: 61 amino acids
Theoretical weight: 6.41 KDa
Source organism: Morganella morganii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56446 (Residues: 12-71; Coverage: 74%)
Gene names: N0392_16430, OSC06_21060, merF
Sequence domains: Membrane transport protein MerF
Structure domains: bacterial mercury transporter, merf

Ligands and Environments

No bound ligands
1 modified residue:
Ligand HSE 1 x HSE

Experiments and Validation Details

Entry percentile scores
Refinement method: DIRECT STRUCTURAL FITTING OF 2D SOLID-STATE NMR DATA
Expression system: Escherichia coli

Quick links

Citations

17 review citations

The magic of bicelles lights up membrane protein structure.
Dürr et al. (2012)
16 more

4 mentions without citation

Membrane protein structure from rotational diffusion.
Das et al. (2015)
3 more