› 2h6r

X-ray diffraction
2.3Å resolution

Crystal Structure of triosephosphate isomerase (TIM) from Methanocaldococcus jannaschii

Released:
DOI: 10.2210/pdb2h6r/pdb
PDB entry 2h6r coloured by chain, front view.
PDB entry 2h6r coloured by chain, side view.
PDB entry 2h6r coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Primary publication:
Structure of triosephosphate isomerase (TIM) from Methanocaldococcus jannaschii.
Gayathri P, Banerjee M, Vijayalakshmi A, Azeez S, Balaram H, Balaram P, Murthy MR
Acta Crystallogr D Biol Crystallogr 63 206-20 (2007)
PMID: 17242514

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate = glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176709 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triosephosphate isomerase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 219 amino acids
Theoretical weight: 23.27 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58923 (Residues: 1-219; Coverage: 100%)
Gene names: MJ1528, tpiA
Sequence domains: Triosephosphate isomerase
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P31
Unit cell:
a: 139.34Å b: 139.34Å c: 80.89Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.213 0.278
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
11 other articles

3 mentions without citation

Tetartohedral twinning could happen to you too.
Roversi et al. (2012)
2 more