› 2h8e

X-ray diffraction
1.2Å resolution

Structure RusA D70N

Released:
DOI: 10.2210/pdb2h8e/pdb
PDB entry 2h8e coloured by chain, front view.
PDB entry 2h8e coloured by chain, side view.
PDB entry 2h8e coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
RusA Holliday junction resolvase: DNA complex structure--insights into selectivity and specificity.
Macmaster R, Sedelnikova S, Baker PJ, Bolt EL, Lloyd RG, Rafferty JB
Nucleic Acids Res 34 5577-84 (2006)
PMID: 17028102

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142795 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Crossover junction endodeoxyribonuclease RusA Chain: A
Molecule details ›
Chain: A
Length: 120 amino acids
Theoretical weight: 13.87 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG74 (Residues: 1-120; Coverage: 100%)
Gene names: JW0538, b0550, rus, rusA, ybcP
Sequence domains: Endodeoxyribonuclease RusA
Structure domains: Holliday junction resolvase RusA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 60.153Å b: 85.107Å c: 57.514Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.183 0.191
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
3 more

2 mentions without citation

Macromolecular ab initio phasing enforcing secondary and tertiary structure.
Millán et al. (2015)
1 more