› 2h8r

X-ray diffraction
3.2Å resolution

Hepatocyte Nuclear Factor 1b bound to DNA: MODY5 Gene Product

Released:
DOI: 10.2210/pdb2h8r/pdb
PDB entry 2h8r coloured by chain, front view.
PDB entry 2h8r coloured by chain, side view.
PDB entry 2h8r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of disease-causing mutations in hepatocyte nuclear factor 1beta.
Lu P, Rha GB, Chi YI
Biochemistry 46 12071-80 (2007)
PMID: 17924661

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115330 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Hepatocyte nuclear factor 1-beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 25.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35680 (Residues: 91-310; Coverage: 40%)
Gene names: HNF1B, TCF2
Sequence domains: Hepatocyte nuclear factor 1 (HNF-1), N terminus
Structure domains:
5'-D(*CP*TP*TP*GP*GP*TP*TP*AP*AP*TP*AP*AP*TP*TP*CP*AP*CP*CP*AP*G)-3' Chain: E
Molecule details ›
Chain: E
Length: 20 nucleotides
Theoretical weight: 6.11 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*GP*CP*TP*GP*GP*TP*GP*AP*AP*TP*TP*AP*TP*TP*AP*AP*CP*CP*AP*A)-3' Chain: F
Molecule details ›
Chain: F
Length: 20 nucleotides
Theoretical weight: 6.16 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: R3
Unit cell:
a: 174.689Å b: 174.689Å c: 72.434Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.223 0.29
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

5 review citations

HNF1B-associated clinical phenotypes: the kidney and beyond.
Bockenhauer et al. (2016)
4 more

6 mentions without citation

New insights into the role of HNF-1β in kidney (patho)physiology.
Ferrè et al. (2019)
5 more