› 2hct

X-ray diffraction
1.95Å resolution

Acidic residues at the active sites of CD38 and ADP-ribosyl cyclase determine NAAPD synthesis and hydrolysis activities

Released:
DOI: 10.2210/pdb2hct/pdb
PDB entry 2hct coloured by chain, front view.
PDB entry 2hct coloured by chain, side view.
PDB entry 2hct coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Acidic residues at the active sites of CD38 and ADP-ribosyl cyclase determine nicotinic acid adenine dinucleotide phosphate (NAADP) synthesis and hydrolysis activities.
Graeff R, Liu Q, Kriksunov IA, Hao Q, Lee HC
J Biol Chem 281 28951-7 (2006)
PMID: 16861223

Function and Biology Details

Reactions catalysed: 
(1a) NADP(+) = 2'-phospho-cyclic ADP-ribose + nicotinamide
(1a) NAD(+) = cyclic ADP-ribose + nicotinamide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151306 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 30.31 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P28907 (Residues: 45-300; Coverage: 85%)
Gene name: CD38
Sequence domains: ADP-ribosyl cyclase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NMN 2 x NMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P1
Unit cell:
a: 41.715Å b: 52.823Å c: 65.578Å
α: 106.26° β: 91.85° γ: 95.25°
R-values:
R R work R free
0.205 0.195 0.248
Expression system: Komagataella pastoris

Quick links

Citations

15 review citations

NAD+ metabolism in health and disease.
Belenky et al. (2007)
14 more

7 mentions without citation

The 2-hydroxycarboxylate transporter family: physiology, structure, and mechanism.
Sobczak et al. (2005)
6 more