› 2hfw

X-ray diffraction
2.5Å resolution

Structural and kinetic analysis of proton shuttle residues in the active site of human carbonic anhydrase III

Released:
DOI: 10.2210/pdb2hfw/pdb
PDB entry 2hfw coloured by chain, front view.
PDB entry 2hfw coloured by chain, side view.
PDB entry 2hfw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and kinetic analysis of proton shuttle residues in the active site of human carbonic anhydrase III.
Elder I, Fisher Z, Laipis PJ, Tu C, McKenna R, Silverman DN
Proteins 68 337-43 (2007)
PMID: 17427958

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139595 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 3 Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 29.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P07451 (Residues: 1-260; Coverage: 100%)
Gene name: CA3
Sequence domains: Eukaryotic-type carbonic anhydrase
Structure domains: Alpha carbonic anhydrase

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 42.373Å b: 51.674Å c: 117.659Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.239
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Structural annotation of human carbonic anhydrases.
Aggarwal et al. (2013)
3 more

1 mention without citation

Acipimox inhibits human carbonic anhydrases.
Mori et al. (2022)