› 2hil

Electron Microscopy
12.5Å resolution

Structure of the Neisseria gonorrhoeae Type IV pilus filament from x-ray crystallography and electron cryomicroscopy

Released:
DOI: 10.2210/pdb2hil/pdb
PDB entry 2hil coloured by chain, front view.
PDB entry 2hil coloured by chain, side view.
PDB entry 2hil coloured by chain, top view.
Source organism: Neisseria gonorrhoeae
Primary publication:
Type IV pilus structure by cryo-electron microscopy and crystallography: implications for pilus assembly and functions.
Craig L, Volkmann N, Arvai AS, Pique ME, Yeager M, Egelman EH, Tainer JA
Mol Cell 23 651-62 (2006)
PMID: 16949362
Related structures: EMD-1236

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136425 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Type IV major pilin protein PilE1 Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R
Length: 158 amino acids
Theoretical weight: 17.21 KDa
Source organism: Neisseria gonorrhoeae
UniProt:
  • Canonical: P02974 (Residues: 8-165; Coverage: 96%)
Gene name: pilE1
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: DT6, GLA
Carbohydrate polymer
1 bound ligand:
Ligand OPE 18 x OPE
1 modified residue:
Ligand MEA 18 x MEA

Experiments and Validation Details

Entry percentile scores
Resolution: 12.5Å
Relevant EMDB volumes: EMD-1236

Quick links

Citations

54 review citations

X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution.
Putnam et al. (2007)
53 more

31 mentions without citation

Surface organelles assembled by secretion systems of Gram-negative bacteria: diversity in structure and function.
Thanassi et al. (2012)
30 more