› 2hor

X-ray diffraction
1.6Å resolution

Crystal structure of alliinase from garlic- apo form

Released:
DOI: 10.2210/pdb2hor/pdb
PDB entry 2hor coloured by chain, front view.
PDB entry 2hor coloured by chain, side view.
PDB entry 2hor coloured by chain, top view.
Source organism: Allium sativum
Primary publication:
Two structures of alliinase from Alliium sativum L.: apo form and ternary complex with aminoacrylate reaction intermediate covalently bound to the PLP cofactor.
Shimon LJ, Rabinkov A, Shin I, Miron T, Mirelman D, Wilchek M, Frolow F
J Mol Biol 366 611-25 (2007)
PMID: 17174334

Function and Biology Details

Reaction catalysed: 
An S-alkyl-L-cysteine S-oxide = an alkyl sulfenate + 2-aminoacrylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alliin lyase 1 Chain: A
Molecule details ›
Chain: A
Length: 427 amino acids
Theoretical weight: 49.06 KDa
Source organism: Allium sativum
UniProt:
  • Canonical: Q01594 (Residues: 39-465; Coverage: 93%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Ligand NAG 2 x NAG
Ligand BMA 1 x BMA
Ligand MAN 2 x MAN
Ligand FUC 1 x FUC
5 bound ligands:
Ligand NAG 1 x NAG
Ligand SO4 5 x SO4
Ligand ACT 1 x ACT
Ligand CL 2 x CL
Ligand NO3 1 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P41212
Unit cell:
a: 81.118Å b: 81.118Å c: 164.017Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.163 0.179

Quick links

Citations

5 review citations

Structural biology of plant sulfur metabolism: from assimilation to biosynthesis.
Ravilious et al. (2012)
4 more

4 mentions without citation

Rapid synthesis of auxin via a new tryptophan-dependent pathway is required for shade avoidance in plants.
Tao et al. (2008)
3 more