› 2hrl

X-ray diffraction
1.85Å resolution

Siglec-7 in complex with GT1b

Released:
DOI: 10.2210/pdb2hrl/pdb
PDB entry 2hrl coloured by chain, front view.
PDB entry 2hrl coloured by chain, side view.
PDB entry 2hrl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Siglec-7 undergoes a major conformational change when complexed with the alpha(2,8)-disialylganglioside GT1b.
Attrill H, Imamura A, Sharma RS, Kiso M, Crocker PR, van Aalten DM
J Biol Chem 281 32774-83 (2006)
PMID: 16895906

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195116 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Sialic acid-binding Ig-like lectin 7 Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 14.66 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q9Y286 (Residues: 18-144; Coverage: 28%)
Gene names: AIRM1, SIGLEC7
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL, SIA, NGA
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand CEQ 1 x CEQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3221
Unit cell:
a: 46.021Å b: 46.021Å c: 106.474Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.195 0.234
Expression system: Cricetulus griseus

Quick links

Citations

17 review citations

Siglecs and their roles in the immune system.
Crocker et al. (2007)
16 more

12 mentions without citation

Current Status on Therapeutic Molecules Targeting Siglec Receptors.
Lenza et al. (2020)
11 more