› 2hw7

X-ray diffraction
2.71Å resolution

Crystal Structure of Mnk2-D228G in complex with Staurosporine

Released:
DOI: 10.2210/pdb2hw7/pdb
PDB entry 2hw7 coloured by chain, front view.
PDB entry 2hw7 coloured by chain, side view.
PDB entry 2hw7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mitogen-activated protein kinases interacting kinases are autoinhibited by a reprogrammed activation segment.
Jauch R, Cho MK, Jäkel S, Netter C, Schreiter K, Aicher B, Zweckstetter M, Jäckle H, Wahl MC
EMBO J 25 4020-32 (2006)
PMID: 16917500

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190755 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MAP kinase-interacting serine/threonine-protein kinase 2 Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 35.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9HBH9 (Residues: 72-385; Coverage: 68%)
Gene names: GPRK7, MKNK2, MNK2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand STU 1 x STU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3221
Unit cell:
a: 102.366Å b: 102.366Å c: 76.439Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.246
Expression system: Escherichia coli BL21

Quick links

Citations

8 review citations

Biochemical aspects of the neuroprotective mechanism of PTEN-induced kinase-1 (PINK1).
Mills et al. (2008)
7 more

18 mentions without citation

Targeting Mnks for cancer therapy.
Hou et al. (2012)
17 more