› 2hzc

X-ray diffraction
1.47Å resolution

Crystal structure of the N-terminal RRM of the U2AF large subunit

Released:
DOI: 10.2210/pdb2hzc/pdb
PDB entry 2hzc coloured by chain, front view.
PDB entry 2hzc coloured by chain, side view.
PDB entry 2hzc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Alternative conformations at the RNA-binding surface of the N-terminal U2AF(65) RNA recognition motif.
Thickman KR, Sickmier EA, Kielkopf CL
J Mol Biol 366 703-10 (2007)
PMID: 17188295

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-150718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Splicing factor U2AF 65 kDa subunit Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 9.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P26368 (Residues: 148-229; Coverage: 17%)
Gene names: U2AF2, U2AF65
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand P6G 1 x P6G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P43212
Unit cell:
a: 29.07Å b: 29.07Å c: 184.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.137 0.139 0.226
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Structure and function of the Pre-mRNA splicing machine.
Sperling et al. (2008)
3 more

1 mention without citation

The dipeptidyl peptidase IV inhibitors vildagliptin and K-579 inhibit a phospholipase C: a case of promiscuous scaffolds in proteins.
Chakraborty et al. (2013)