› 2i7t

X-ray diffraction
2.1Å resolution

Structure of human CPSF-73

Released:
DOI: 10.2210/pdb2i7t/pdb
PDB entry 2i7t coloured by chain, front view.
PDB entry 2i7t coloured by chain, side view.
PDB entry 2i7t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Polyadenylation factor CPSF-73 is the pre-mRNA 3'-end-processing endonuclease.
Mandel CR, Kaneko S, Zhang H, Gebauer D, Vethantham V, Manley JL, Tong L
Nature 444 953-6 (2006)
PMID: 17128255

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193845 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cleavage and polyadenylation specificity factor subunit 3 Chain: A
Molecule details ›
Chain: A
Length: 459 amino acids
Theoretical weight: 52.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UKF6 (Residues: 1-459; Coverage: 67%)
Gene names: CPSF3, CPSF73
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 58.834Å b: 82.595Å c: 103.705Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.231 0.276
Expression system: Escherichia coli

Quick links

Citations

89 review citations

RNA-binding proteins and post-transcriptional gene regulation.
Glisovic et al. (2008)
88 more

17 mentions without citation

Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.
Xiang et al. (2014)
16 more