› 2iak

X-ray diffraction
3Å resolution

Crystal Structure of a protease resistant fragment of the plakin domain of Bullous Pemphigoid Antigen1 (BPAG1)

Released:
DOI: 10.2210/pdb2iak/pdb
PDB entry 2iak coloured by chain, front view.
PDB entry 2iak coloured by chain, side view.
PDB entry 2iak coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural analysis of the plakin domain of bullous pemphigoid antigen1 (BPAG1) suggests that plakins are members of the spectrin superfamily.
Jefferson JJ, Ciatto C, Shapiro L, Liem RK
J Mol Biol 366 244-57 (2007)
PMID: 17161423

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-187627 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dystonin Chain: A
Molecule details ›
Chain: A
Length: 224 amino acids
Theoretical weight: 26.56 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q91ZU6 (Residues: 580-803; Coverage: 3%)
Gene names: Bpag1, Dst, Macf2
Sequence domains: Spectrin repeat
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I4132
Unit cell:
a: 195.8Å b: 195.8Å c: 195.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.267
Expression system: Escherichia coli BL21

Quick links

Citations

17 review citations

Desmosome structure, composition and function.
Garrod et al. (2008)
16 more

6 mentions without citation

The structure of the ankyrin-binding site of beta-spectrin reveals how tandem spectrin-repeats generate unique ligand-binding properties.
Stabach et al. (2009)
5 more