› 2iau

X-ray diffraction
2Å resolution

Crystal structure of squid ganglion DFPase W244Y mutant

Released:
DOI: 10.2210/pdb2iau/pdb
PDB entry 2iau coloured by chain, front view.
PDB entry 2iau coloured by chain, side view.
PDB entry 2iau coloured by chain, top view.
Source organism: Loligo vulgaris
Primary publication:
Crystal structure of diisopropylfluorophosphatase from Loligo vulgaris.
Scharff EI, Koepke J, Fritzsch G, Lücke C, Rüterjans H
Structure 9 493-502 (2001)
PMID: 11435114

Function and Biology Details

Reaction catalysed: 
Diisopropyl fluorophosphate + H(2)O = diisopropyl phosphate + fluoride
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181980 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diisopropyl-fluorophosphatase Chain: A
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 34.84 KDa
Source organism: Loligo vulgaris
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7SIG4 (Residues: 3-314; Coverage: 99%)
Sequence domains: SMP-30/Gluconolactonase/LRE-like region
Structure domains: TolB, C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 42.901Å b: 81.696Å c: 86.246Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 0.264
Expression system: Escherichia coli BL21

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Citations

14 review citations

Emergence of diverse biochemical activities in evolutionarily conserved structural scaffolds of proteins.
Anantharaman et al. (2003)
13 more